DNA-based data storage is a potential alternative to traditional storage media, with potential utility for living biosensors. However, the majority of existing DNA recorders possess low storage capacities and limited temporal-sequential recording capabilities. Moreover, current DNA recorders also lack simple, efficient, and reliable single-step methods for converting electronic data into DNA.

In this project, an optogenetic controlled DNA recorder was developed. It uses CRISPR Cas1-Cas2 for a natural cellular DNA ticker-tape which allows for temporal, sequential recording with theoretically unlimited capacities. It uses an EL222 blue-light regulated system for instantaneous control of digital electronic to DNA data encoding.

Project Team

Student:

• Lin Zhangyuan (Biomedical Engineering, Class of 2023)

Supervisor:

• Poh Chueh Loo (biepcl@nus.edu.sg)