Dr. Andrew W. HOLLE

Education

• Ph.D. in Bioengineering, University of California, San Diego, USA
• B.S.E. in Biomedical Engineering (Statistics Minor), Arizona State University, USA

Research Interest

1. Stem Cell Mechanobiology
2. Cytoskeletal Dynamics
3. Cancer Migration and Invasion
4. Microfluidic devices

Selected Awards & Honours

1. NRF Fellow, National Research Foundation Singapore, 2021-2026
2. American Association for Cancer Research (AACR) Basic Research Fellow, 2016-2017
3. American Association for Cancer Research (AACR) Scholar-in-Training Award, 2016
4. American Society for Cell Biology (ASCB) Postdoc Travel Award, 2015
5. The European Association for Cancer Research (EACR) Travel Award, 2015
6. Max Planck Institute Postdoctoral Fellowship, 2014-2017
7. Chancellor’s Dissertation Medal, Department of Bioengineering Nominee, University of California, San Diego, 2013
8. ASME SBC PhD Student Paper Competition, Second Place, 2013
9. Shu Chien NSF-BMES Award for Excellence in Mechanobiology and Mechanotransduction, 2011

Membership in Scientific/Professional Organization

• Biomedical Engineering Society
• International Society for Stem Cell Research
• Biophysical Society
• American Society for Cell Biology

Selected Journal Publications

1. Wei, Q., Holle, A.W., Li, J., Posa, F., Biagioni, F., Crocii, O., Benk, A., Young, J., Noureddine, F., Deng, J., Zhang, M., Inman, G.J., Spatz, J.P., Campaner, S., Cavalcanti-Adam, E.A. “BMP-2 signaling and mechanotransduction synergize to drive osteogenic differentiation via YAP/TAZ” Advanced Science 1902931 (2020)
2. Wei, Q., Young, J.L., Holle, A.W., Li, J., Bieback, K., Inman, G., Spatz, J.P., Cavalcanti-Adam, E.A. “Soft Hydrogels for Balancing Cell Proliferation and Differentiation” ACS Biomaterials Science & Engineering 6, 4687-4701 (2020)
3. Yu, L., Hou, Y., Xie, W., Camacho, L.C., Cheng, C., Holle, A.W., Young, J., Trappmann, B., Zhao, W., Melzig, M.F., Cavalcanti-Adam, E.A., Zhao, C., Spatz, J.P., Wei, Q., Haag, R. “Integrin α5β1and Rac signaling promote cell adhesion in a contractility-independent manner on dynamic ligand surfaces” 32, 2002566 Advanced Materials (2020)
4. Kadiri, V.M., Bussi, C., Holle, A.W., Son, K., Kwon, H., Schütz, G., Gutierrez, M., Fischer, P. Transfection of cells via active transport of magnetic FePt nanopropellers Advanced Materials 2001114 (2020)
5. Kim, C., Young, J.L., Holle, A.W., Jeong, K., Major, L., Jeong, J.H., Aman, Z., Han, D.W., Hwang, Y., Spatz, J.P., Choi, Y.S. “Stem cell mechanosensation on gelatin methacryloyl (GelMA) stiffness gradient hydrogels” Annals of Biomedical Engineering 48, 893-902 (2020)
6. Ma, Z., Holle, A.W., Melde, K., Qiu, T., Peoppel, K., Kadiri, V.M., Fischer, P. “Acoustic Holographic Cell Patterning in a Biocompatible Hydrogel” Advanced Materials 32, 1904181 (2020)
7. Major, L.G.*, Holle, A.W.*, Young, J.L.*, Chin, I., Hepburn, M., Sanderson, R., Kennedy, B., Spatz, J.P., Han, D.W., Hwang, Y., Park, H.W., Guan, K.L., Choi, Y.S. “Volume adaptation controls stem cell mechanotransduction” ACS Applied Materials and Interfaces 11, 45520-45530 (2019) *Equal contribution
8. Holle, A.W., and Kemkemer, R. “Molecular motors: stronger than they look” Nature Physics 15, 628-629 (2019)
9. Holle, A.W., Govindankutty Devi, N., Clar, K., Fan, A., Kemkemer, R., Spatz, J.P. “Cancer cells invade confined microchannels via a self-directed mesenchymal-to-amoeboid transition” Nano Letters 19, 2280-2290 (2019)
10. Holle, A.W., Young, J.L., Van Vliet, K.J., Kamm, R.D., Discher, D., Janmey, P., Spatz, J.P., Saif, T. “Cell-ECM Mechanobiology- forceful tools and emerging needs for basic and translational research” Nano Letters 18, 1-8 (2018)
11. Sales Ramos, A., Holle, A.W., Kemkemer, R. “Initial contact guidance during cell spreading is contractility-independent” Soft Matter 13, 5158-5167 (2017)
12. Hadden, W.*, Young, J.L.*, Holle, A.W.*, McFetridge, M., Kim, D.Y., Wijesinghe, P., Taylor-Weiner, H., Wen, J.H., Lee, A.R., Bieback, K., Vo, B.N., Sampson, D.D., Kennedy, B.F., Spatz, J.P., Engler, A.J., Choi, Y.S. “Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels” PNAS 114, 5647-5652 (2017) *Equal contribution
13. Holle, A.W., Kalafat, M., Sales Ramos, A., Seufferlein, T., Kemkemer, R., Spatz, J.P. “Intermediate filament reorganization dynamically influences cancer cell alignment and migration” Scientific Reports 7, 45152 (2017)
14. Holle, A.W., McIntyre, A.J., Kehe, J., Wijesekara, P., Young, J.L., Vincent, L.V., Engler, A.J. “High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation” Integrative Biology 8, 1049-1058 (2016)
15. Holle, A.W., Young, J.L., Spatz, J.P. “In vitro cancer cell-ECM interactions inform in vivo cancer treatment” Advanced Drug Delivery Reviews 97, 270-279 (2015)
16. Young, J.L., Holle, A.W., Spatz, J.P. “Nanoscale and mechanical properties of the physiological cell-ECM microenvironment” Experimental Cell Research 343, 3-6 (2016)
17. Holle, A.W., Young, J.L, and Choi, Y.S. “Forces of Nature: Understanding the role of mechanotransduction in stem cell differentiation” Integrative Mechanobiology 205-226 (2015)
18. Holle, A.W., Tang, X., Vijayraghavan, D., Vincent, L.G., Fuhrmann, A., Choi, Y.S., del Alamo, J.C., and Engler, A.J. “In situ mechanotransduction via vinculin regulates stem cell differentiation” Stem Cells 31, 2467-2477 (2013)
19. Choi, Y.S., Holle, A.W., and Engler, A.J. “Engineered ECM microenvironments and their regulation of stem cells” Extracellular Matrix in Development 133-160 (2013)
20. Holle, A.W. and Engler, A.J. “More than a feeling: discovering, understanding, and influencing mechanosensitive pathways” Current Opinion in Biotechnology 22, 648-654 (2011)
21. Holle, A.W. and Engler, A. J. “Cell rheology: stressed-out stem cells” Nature Materials 9, 4-6 (2010)