Biomedical Optical Spectroscopy (Raman, Fluorescence and Diffuse Reflectance) and Imaging in Biomedicine
Advanced Fiber-Optic Endoscopic Imaging
Coherent Raman Scattering Microscopy Imaging
Multimodal Nonlinear Optical Microscopy Imaging
Nonlinear Fluorescence Lifetime Imaging
Teaching Modules
BN2101 Principles of Bioengineering
BN2203 Introduction to Bioengineering Design
BN3401 Biomedical Electronics and Systems
BN4406 Biophotonics and Bioimaging
GEK2505 Introductory Biomedical Engineering
BN5207 Medical Imaging Systems
Selected Journal Publications
K Bae, W Zheng, Y Ma, Z Huang*, Real-time monitoring of pharmacokinetics of mitochondria-targeting molecules in live cells with bioorthogonal hyperspectral stimulated Raman scattering microscopy, Analytical Chemistry, 2019 (in press), https://doi.org/10.1021/acs.analchem.9b02838
L Gong, W Zheng, Y Ma, Z Huang*,  Higher-Order Coherent Anti-Stokes Raman Scattering (HO-CARS) Microscopy Realizes Label-Free Super-Resolution Vibrational Imaging, Nature Photonics, 2019. https://doi.org/10.1038/s41566-019-0535-y
Žuvela, Petar; Lin, Kan; Shu, Chi; Zheng, Wei; Lim, Chwee Ming; Huang, Zhiwei*, Fiber-optic Raman Spectroscopy With Nature-inspired Genetic Algorithms Enhances Real-time In Vivo Detection And Diagnosis of Nasopharyngeal Carcinoma”, Analytical Chemistry. 91 (13), 8101-8108 (2019); https://doi.org/10.1021/acs.analchem.9b00173
Bae Kideog, Wei Zheng, Ying Ma, and Zhiwei Huang*, Real-time monitoring of pharmacokinetics of antibiotics in biofilms with Raman-tagged hyperspectral stimulated Raman scattering microscopy, Theranostics 2019; 9(5):1348-1357. doi:10.7150/thno.32043
Li Gong, Jian Lin, Chenglong Hao, Wei Zheng, Steve Qing Yang Wu, Jinghua Teng, Cheng-wei Qiu, Zhiwei Huang*, Supercritical Focusing Coherent anti-Stokes Raman scattering microscopy for high-resolution vibrational imaging, Optics Letters, 43 (23), 5615-5618(2018)  https://doi.org/10.1364/OL.43.005615
Bae Kideog, Wei Zheng, Kan Lin, Carol Tang, Christopher Beng Ti Ang, and Zhiwei Huang*, Epi-detected hyperspectral stimulated Raman scattering microscopy for label-free molecular subtyping of glioblastomas, Anal. Chem., 90 (17): 10249-10255 (2018).   2018 Aug 2. doi: 10.1021/acs.analchem.8b01677.
Wang FH, Bae K, Huang Z, Xue JM, Two-photon graphene quantum dot modified Gd2O3 nanocomposites as a dual-mode MRI contrast agent and cell labelling agent, Nanoscale 10(12):5642-5649 (2018).  DOI: 10.1039/c7nr08068a
J Wang, K Lin, W Zheng, Z Huang*, Characterizing biochemical and morphological variations of clinically relevant anatomical locations of oral tissue in vivo with hybrid Raman spectroscopy and optical coherence tomography technique, J. Biophotonics 11(3):01-09 (2018).  DOI: 10.1002/jbio.201700113.
K Goda, A Zumbusch, Z Huang*, Y Ozeki, Guest Editorial: Special Topic on Coherent Raman Spectroscopy and Imaging, APL Photonics 3 (9), 090401 (2018). https://doi.org/10.1063/1.5052267
K Bae, W Zheng, and ZÂ Huang*, Quantitative assessment of spinal cord injury using circularly polarized coherent anti-Stokes Raman scattering microscopy, Appl. Phy. Lett., 111, 063704 (2017); doi: 10.1063/1.4991792.
K Lin, W Zheng, C Lim, ZÂ Huang*, Real-time in vivo diagnosis of nasopharyngeal carcinoma using rapid fiber-optic Raman spectroscopy, Theranostics, 7(14):3517-3526 (2017). doi:10.7150/thno.16359.
Y Ye, F Xu, G Wei, Y Xu, D Pu, L Chen, ZÂ Huang*, Scalable Fourier transform system for instantly structured illumination in lithography, Optics Letters, 42(10), 1978- 1981 (2017).
C Lim, R Nagaraja, T Loh, W Zheng, J Wang, K Lin, ZÂ Huang*, Real time near-infrared Raman spectroscopy for the diagnosis of nasopharyngeal cancer, Oncotarget, 2017, 1-8, doi: 10.18632/oncotarget.17703.
K Lin, W Zheng Wei, CM Lim, Z Huang*, Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman endoscopy, Biomedical Optics Express, 7 (9), 3705-3715 (2016).
Z Wang, W Zheng, ZÂ Huang*, Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging, Optics Letters, 41 (17), 3960-3963 (2016).
J Wang, W Zheng, K Lin, Z Huang*, Development of a hybrid Raman spectroscopy and optical coherence tomography technique for real-time in vivo tissue measurements, Optics Letters, 41 (13), 3045-3048 (2016).
K Lin, J Wang, W Zheng, K Ho, M Teh, K Yeoh, Z Huang*, Rapid fiber-optic Raman spectroscopy for real-time in vivo detection of gastric intestinal metaplasia during clinical gastroscopy, Cancer Prevention Research, 2016; doi: 10.1158/1940-6207.CAPR-15-0213.
J Wang, W Zheng, K Lin, and Z Huang*, Integrated Mueller-Matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection, Biomedical Optics Express, 7 (4), 1116-1126 (2016). doi: 10.1364/BOE.7.001116.
Z Wang, W Zheng, CY Hsu, and Z Huang*, Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy, Biomedical Optics Express, 7(4), 1284-1293 (2016). DOI:10.1364/BOE.7.001284.
Z Wang, W Zheng, CY Hsu, and Z Huang*, Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth, Appl. Phys. Lett., 108, 033701 (2016); doi: 10.1063/1.4939923.
J Wang, K Lin, W Zheng, K Ho, M Teh, K Yeoh, Z Huang*, Fiber-optic Raman spectroscopy for in vivo diagnosis of gastric dysplasia, Faraday Discuss., 2015, DOI: 10.1039/C5FD00151J
Z Dong, M Asbahi, J Lin, D Zhu, Y Wang, K Hippalgaonkar, H Chu, W Goh, F Wang, Z Huang*, and J Yang*, Second-Harmonic Generation from Sub-5 nm Gaps by Directed Self-Assembly of Nanoparticles onto Template-Stripped Gold Substrates, Nano Lett., 2015. DOI: 10.1021/acs.nanolett.5b02109.
J Wang, K Lin, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy, Scientific Reports, 5, 12957; DOI: 10.1038/srep12957 (2015).
J Wang, K Lin, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Comp arative study of the endoscope-based bevelled and volume fiber-optic Raman probes for optical diagnosis of gastric dysplasia in vivo at endoscopy. Anal. Bioanal. Chem., (2015); DOI:10.1007/s00216-015-8727-x.
M Bergholt, K Lin, J Wang, W Zheng, H Xu, Q Huang, JL Ren, KY Ho, M Teh, S Srivastava, B Wong, KG Yeoh, Z Huang* , Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances real-time in vivo diagnosis of adenomatous polyps during colonoscopy. J. Biophotonics,(2015); DOI: 10-1002/jbio.201400141.
Z Wang, W Zheng, CY Hsu, and Z Huang*, Epi-detected quadruple-modal nonlinear optical microscopy for label-free tooth imaging, Appl. Phys. Lett., 106, 033701 (2015).
M Bergholt, W Zheng, K Lin, J Wang, H Xu, J Ren, K Ho, M Teh, K Yeoh, Z Huang*, Characterizing variability of in vivo Raman spectroscopic properties of different anatomical sites of normal colorectal tissue towards cancer diagnosis at colonoscopy”, Anal. Chem., 87, 960-966 (2015).
J Lin, W Zheng, Z Wang, and Z Huang*, Label-free three-dimensional imaging of cell nucleus using third-harmonic generation microscopy, Appl. Phys. Lett. 105, 103705 (2014).
S. Duraipandian, J Mo, W. Zheng, and Z. Huang*, “Near-infrared Raman spectroscopy for assessing biochemical changes of cervical tissue associated with precarcinogenic transformation” Analyst, 139 (21), 5379-5386 (2014).
J Lin, SK Teh, W Zheng, Z Wang, Z Huang*, Multimodal nonlinear optical microscopic imaging provides new insights into acetowhitening mechanisms in live mammalian cells without labelling, Biomedical Optics Express, 5(9), 3116-3122 (2014).
M Bergholt, W Zheng, KY Ho, M Teh,  KG Yeoh, J So, A Shabbir, Z Huang*, Fiber-optic confocal Raman spectroscopy for real-time in vivo diagnosis of dysplasia in Barrett’s esophagus,  Gastroenterology, 146(1), 27-32 (2014).
M Bergholt, S Duraipandian, W Zheng, Z Huang*, Multivariate reference technique for quantitative analysis of fiber-optic tissue Raman spectroscopy, Anal. Chem. 85, 11297-11303 (2013).
J Lin, S Pan, W Zheng, Z Huang*, Polarization-resolved second-harmonic generation imaging for liver fibrosis assessment without labeling, Appl. Phys. Lett. 103, 173701 (2013).
M Bergholt, W Zheng, KY Ho, M Teh,  KG Yeoh, J So, A Shabbir, Z Huang*, Raman endoscopy for objective diagnosis of early cancer in the gastrointestinal system, Journal of Gastrointestinal & Digestive System, 2013, S:1. http://dx.doi.org/10.4172/2161-069X.S1-008.
J Lin, K Er, W Zheng, Z Huang*,  Radially polarized tip-enhanced near-field coherent anti-Stokes Raman scattering microscopy for vibrational nano-imaging, Appl. Phys. Lett., 103, 083705 (2013)
J Wang, M Bergholt, W Zheng, Z Huang*, Development of a beveled fiber-optic confocal Raman endoscopic probe for enhancing in vivo epithelial tissue Raman measurements at endoscopy. Optics Letters, 38 (13), 2321-2323 (2013).
S Duraipandian, W Zheng, J Ng, J.H. Low, A. Ilancheran, and Z Huang*, Near-infrared-excited confocal Raman spectroscopy advances in vivo detection of cervical precancer, J. Biomed. Opt. 18 (6), 067007 (2013).
S Duraipandian, W Zheng, J Ng, J.H. Low, A. Ilancheran, and Z Huang*, Noninvasive analysis of hormonal variations and vagifem treatment effect on postmenopausal women using in vivo high wavenumber confocal Raman spectroscopy, Analyst, 138 (14), 4120-4128 (2013).
P Upputuri, J Lin, G Li, X Liu, H Wang*, Z Huang*,  Circularly Polarized Coherent Anti-Stokes Raman Scattering Microscopy,  Optics Letters, 38(8),  1262-1264 (2013).
M Bergholt, W Zheng, Z Huang*, Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy, Journal of Biomedical Optics, 18 (3), 30502 (2013).
M Bergholt, W Zheng, KY Ho, M Teh, KG Yeoh, J So, A Shabbir, Z Huang*, Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy, J. Biophotonics Vol.6, No.1, 49-59 (2013) DOI 10.1002/jbio.201200138.
Men-Qiao Dai, Wei Zheng, Zhiwei Huang and Lin-Yue Lanry Yung, Aqueous phase synthesis of widely tunable photoluminescence emission CdTe/CdS core/shell quantum dots under a totally ambient atmosphere, J. Mater. Chem. 22 (32), 16336 – 16345 (2012)
M Bergholt, W Zheng, Z Huang*, Characterizing variability in in vivo Raman spectroscopic properties of different anatomical sites of normal tissue in the oral cavity, Journal of Raman Spectroscopy, 43, 255-262 (2012).
K Lin, D Lau, Z Huang*, Optical diagnosis of laryngeal cancer using high wavenumber Raman spectroscopy, Biosensors and Bioelectronics 35, 213-217 (2012).
S Duraipandian, W Zheng, J Ng, Jeffrey Low, A Ilanacheran, Z Huang*, 
Simultaneous fingerprint and high wavenumber confocal Raman spectroscopy enhances early detection of cervical precancer in vivo, Analytical Chemistry, 84, 5913-5919 (2012).
M Bergholt, K Lin, W Zheng, D Lau, Z Huang*, In vivo real-time transnasal image-guided Raman endoscopy: defining spectral properties in the nasopharynx and larynx, Journal of Biomedical Optics, 17 (7), 077002 (2012).
S Duraipandian, M Bergholt, W Zheng, K Ho, M Teh, K Yeoh, J So, A Shabbir, Z Huang*, Real-time biomedical Raman spectroscopy for in vivo, on-line gastric cancer diagnosis during clinical endoscopic examination, Journal of Biomedical Optics, 17 (8), 081418Â (2012).
6. J Lin , F Lu , W Zheng , S Xu , D Tai , H Yu , Z Huang*, Assessment of liver steatosis and fibrosis in a bile duct ligation rat model using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique, Journal of Biomedical Optics, 16(11), 116024 (2011).
X Shao, W Zheng, Z Huang*, Near-infrared autofluorescence spectroscopy for in vivo identification of hyperplastic and adenomatous polyps in the colon, Biosensors and Bioelectronics, 30, 118-122(2011).
S Duraipandian, W Zheng, J Ng, Jeffrey Low, A Ilanacheran, Z Huang*, In vivo diagnosis of cervical precancer using Raman spectroscopy and genetic algorithm techniques, Analyst, 136, 4328-4336 (2011).
B Lin, M Bergholt, D Lau, Z Huang*, Optical diagnosis of nasopharyngeal cancer using ultraviolet autofluorescence excitation-emission matrix spectroscopy and parallel factor analysis, Analyst, 136, 3896-3903 (2011).
X Shao, W Zheng, Z Huang*, In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling, Journal of Biomedical Optics, 16(6), 067005 (2011).
M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, Combining near-infrared-excited autofluorescence and Raman spectroscopy improves in vivo diagnosis of gastric cancer, Biosensors and Bioelectronics, 26, 4104-4110 (2011).
M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, In vivo diagnosis of esophageal cancer using image-guided Raman endoscopy and biomolecular modeling, Technology in Cancer Research & Treatment (TCRT), 10 (2), 103-112 (2011).
M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, Characterizing variability in in vivo Raman spectra of different anatomical sites in the upper gastrointestinal tract toward cancer detection, Journal of Biomedical Optics, 16(3), 037003 (2011).
N Lim, Z Hamed, C Yeow, C Chan, Z Huang*, Early detection of biomolecular changes in disrupted porcine cartilage using polarized Raman spectroscopy, Journal of Biomedical Optics, 16 (1), 017003 (2011).
M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques, Int J Cancer, 128(11), 2673-2680 (2011).
X Shao, W Zheng, Z Huang*, Polarized near-infrared autofluorescence imaging combined with near-infrared diffuse reflectance imaging for improving colonic cancer detection, Optics Express, 18 (23), 24293-24300 (2010).
M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, J So, Z Huang*, Raman endoscopy for in vivo differentiation between benign and malignant ulcers in the stomach, Analyst, 135 (12), 3162–3168 (2010).
Lin, F Lu, W Zheng, Z Huang*, Annular aperture-detected coherent anti-Stokes Raman scattering microscopy for high contrast vibrational imaging, Applied Physics Letters, 97 (8), 083701 (2010).
C Yuen, W Zheng, Z Huang*, Low-level detection of anti-cancer drug in blood plasma using microwave-treated gold-polystyrene beads as surface-enhanced Raman scattering substrates, Biosensors and Bioelectronics, 26, 580-584 (2010).
Z Huang*, SK Teh, W Zheng, K Lin, KY Ho, M Teh, KG Yeoh, In vivo detection of epithelial neoplasia in the stomach using image-guided Raman endoscopy, Biosensors and Bioelectronics, 26, 383-389 (2010).
K Lin, W Zheng, Z Huang*, Integrated autofluorescence endoscopic imaging and point-wise spectroscopy for real-time in vivo tissue measurements, Journal of Biomedical Optics, 15 (4), 040507 (2010).
C Fang, F Lu, W Zheng, Z Huang*, Triple-frequency symmetric subtraction scheme for nonresonant background suppression in coherent anti-Stokes Raman scattering (CARS) microscopy, Optics Express, 18 (15), 15714-15724 (2010).
J Mo, W Zheng, Z Huang*, Fiber-optic Raman probe couples ball lens for depth-selected Raman measurements of epithelial tissue, Biomedical Optics Express, 1 (1), 17-30 (2010).
Z Huang*, M Bergholt, W Zheng, K Lin, K Ho, M Teh, K Yeoh, In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy, Journal of Biomedical Optics, 15 (3), 037017 (2010).
J Lin, W Zheng, H Wang, Z Huang*, Effects of scatterers’ sizes on near-field coherent anti-Stokes Raman scattering under tightly focused radially and linearly polarized light excitation, Optics Express, 18(10), 10888-10895 (2010).
F Lu, W Zheng, J Lin, Z Huang*, Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser, Applied Physics Letters, 96(14), 133701 (2010)
H Li, W Zheng, Z Huang*, Rapid near-infrared fluorescence excitation-emission matrix spectroscopy for multifluorophores characterization using an acousto-optic tunable filter technique, Journal of Biomedical Optics, 15 (2), 027010 (2010).
SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Near-infrared Raman spectroscopy for early diagnosis and typing of adenocarcinoma in the stomach, British Journal of Surgery, 97(4), 550-557 (2010).
SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Near-infrared Raman spectroscopy for optical diagnosis in the stomach: Identification of Helicobacter-pylori infection and intestinal metaplasia, Int. J. Cancer, 126 (8), 1920-1927 (2010).
C Yuen, W Zheng, Z Huang*, Optimization of extinction efficiency of gold-coated polystyrene beads substrates improves surface-enhanced Raman scattering effects by post-growth microwave heating treatment, J. Raman Spectroscopy, 41 (4), 374-380 (2010).
J Mo, W Zheng, Jeffrey Low, J Ng, A Ilanacheran, Z Huang*, High wavenumber Raman spectroscopy for in vivo detection of cervical dysplasia, Analytical Chemistry, 81, 8908-8915 (2009).
J Lin, F Lu, H Wang, W Zheng, C Sheppard, Z Huang*, Improved contrast radially polarized coherent anti-Stokes Raman scattering microscopy using annular aperture detection, Applied Physics Letters, Vol. 95, No. 13, 133703 (2009).
F Lu, W Zheng, Z Huang*, Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light, Optics Letters, Vol.34, No.12, 1870-1872 (2009).
Z Huang*, SK Teh, W Zheng, J Mo, K Lin, X Shao, KY Ho, M Teh, KG Yeoh, Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy, Optics Letters, Vol.34, No.6, 758-760 (2009).
J Lin, H Wang, W Zheng, F Lu, C Sheppard, Z Huang*, Numerical study of effects of light polarization, scatterer sizes and orientations on near-field coherent anti-Stokes Raman scattering microscopy, Optics Express, 17 (4), 2423-2434 (2009).
SK Teh, W Zheng, David P Lau, Z Huang*, Spectroscopic diagnosis of laryngeal carcinoma using near-infrared Raman spectroscopy and random recursive partitioning ensemble techniques, Analyst, 134, 1232-1239 (2009).
SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Near-infrared Raman spectroscopy for gastric precancer diagnosis, J. Raman Spectroscopy, 40, 908-914 (2009).
F Lu, W Zheng, Z Huang*, Elliptically polarized coherent anti-Stokes Raman scattering microscopy, Optics Letters, 33 (23), 2842-2844 (2008).
F Lu, W Zheng, Z Huang*, Phase-controlled polarization coherent anti-Stokes Raman scattering microscopy for high sensitive and high contrast molecular imaging, Journal of the Optical Society of America B, 25 (11), 1907-1913 (2008).
C Yuen, W Zheng, Z Huang*, Improving surface-enhanced Raman scattering effect using gold-coated hierarchical polystyrene bead substrates modified with post-growth microwave treatment, Journal of Biomedical Optics, 13 (6), 064040 (2008).
C Yuen, W Zheng, and Z Huang*, Surface-enhanced Raman scattering: principles, nanostructures, fabrications and biomedical applications, J. Innovative Optical Health Sciences (JIOHS), 1 (2), 267-284 (2008).
F. Jaillon, W. Zheng, and Z Huang*, Half-ball lens couples a beveled fiber probe for depth-resolved spectroscopy: Monte Carlo simulations, Appl. Opt. 47, 3152-3157 (2008).
F Lu, W Zheng, and Z Huang*, Heterodyne polarization coherent anti-Stokes Raman scattering microscopy, Applied Physics Letters, 92 (12), 123901 (2008).
F Lu, W Zheng, C Sheppard, Z Huang*, Interferometric polarization coherent anti-Stokes Raman scattering microscopy, Optics Letters, 33(6), 602-604 (2008).
F Jaillon W Zheng, Z Huang*, Beveled fiber-optic probe couples a ball lens for improving depth-resolved fluorescence measurements of layered tissue: Monte Carlo Simulations, Phys. Med. Biol. 53, 937-951 (2008).
SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Diagnostic potential of near-infrared Raman spectroscopy in the stomach: differentiating dysplasia from normal tissue. British Journal of Cancer, 98, 457-465 (2008).
SK Teh, W Zheng, KY Ho, M Teh, KG Yeoh, Z Huang*, Diagnosis of gastric cancer using near-infrared Raman spectroscopy and classification and regression tree techniques, Journal of Biomedical Optics, 13 (3), 034013 (2008).
E Widjaja, W Zheng and Z Huang*, Classification of colonic tissues using near-infrared Raman spectroscopy and support vector machines. Int. J. Oncology, 32: 653-662 (2008).
